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Week-1 Acitivities

Replicate 1: (morning camp)
Check-in:       8:45 - 9:00am Everyday
Check-out:     12:00pm Everyday

Replicate 2: (Afternoon camp)
Check-in:       12:45-1:00pm Everyday
Check-out:     4:00pm Everyday

Instructors:

 

Week-1

●     Pankaj Jaiswal

●     Justin Preece
●    
Matthew (Matt) Geniza
●    
Noor Al-Bader
●    
Austin Meier
●    
Parul Gupta

●     Jeff Chang

●     Alexandra Weisberg
●    
Elizabeth Savory
●    
Edward Davis

●     Sushma Naithani

●     Jeff Anderson

●     Megan O’Malley

●     Ramesh Sagili

●     Hannah Lucas

 


SCHEDULED ACTIVITIES

Day & Date

Morning Camp

Afternoon Camp

Activity

Lead / Instructors

Location

Day-1

Mon

June 27, 2016

9:00 - 9:15

1:00 - 1:15

Camp Overview

Jaiswal

Cordley 3089

9:15 - 9:45

1:15 - 1:45

Safety and Pipetting (using dyes)

Jaiswal/ Matt

9:45 -10:45

1:45 -2:45

Central Dogma and Genetic Material

(hands on activity on DNA modeling exercise, transcription and translation)

 

Jaiswal/ Noor, Matt

Austin, Parul

10:45 - 11:00

2:45 - 3:00

Break and Snacks

 

Cordley 3086

11:00 - 12:00

3:00 - 4:00

Talk to them about ...What they are doing and why?

 

Genetic transformation of experimental model, tobacco plants, with T-DNA inserts carrying GUS reporter gene (GMOs).

If time permits use the time to discuss GMOs

Chang / Elizabeth

Cordley 3089

 

Day-2

Tue

June 28, 2016

9:00 - 9:05

1:00 - 1:05

Review of Day 1 & Questions?

Jaiswal

Cordley 3089

9:05 - 9:45

1:05 - 1:45

Pipetting (lesson two)

Jaiswal / Matt, Noor, Parul, Austin

9:45 - 10:45

1:45 - 2:45

Plant DNA isolation

Jaiswal / Matt, Noor, Parul, Austin

10:45 - 11:00

2:45 - 3:00

Break and Snacks

 

Cordley 3086

11:00 -12:00

3:00 - 4:00

Bacterial DNA isolation

Chang / Alex,

Ed

Cordley 3089

 

Day-3

Wed

June 29, 2016

9:00 -9:05

1:00 -1:05

Review of Day 2 & Questions?

Alex, Matt,

Noor

Cordley 3089

9:05 - 9:30

1:05 - 1:30

Polymerase Chain Reaction (PCR). Amplify genetic markers (SSR) containing DNA segment from various varietis of rice.

Jaiswal / Matt, Noor, Parul, Austin

9:30 - 10:15

1:30 - 2:15

Preparation of bacterial DNA library for whole genome sequencing

Chang / Alex & Ed

Cordley 3089

10:15 - 10:30

2:15 - 2:30

Break and Snacks

 

Cordley 3086

10:30 - 12:00

2:30 - 4:00

Genetic Markers and Phenotypes (Hands on activity on Mendelian Genetics)

Naithani/ Noor, Matt, Parul, Austin

Cordley 3089

 

Day-4

Thu

June 30, 2016

9:00 - 9:05

1:00 -1:05

Review of Day 3 & Questions?

Alex, Matt, Austin

Cordley 3089

9:05 - 9:30

1:05 - 1:30

GUS reporter gene assay for genetic transformation of tobacco plants (continue from Day-1)

Chang / Elizabeth

9:30 - 10:00

1:30 - 2:00

 

PCR gel loading

Jaiswal / Matt, Noor, Parul, Austin

10:00 - 10:30

2:00 - 2:30

Tour of the Center for Genome Research and Biocomputing (CGRB)-

Form 2 groups

Jaiswal (group-1) /

Noor (group-2)/

Parul

ALS-CGRB

10:30 - 10:45

2:30 - 2:45

Break and Snacks

 

Cordley 3086

10:45 - 12:00

2:45 - 4:00

DNA Modeling Exercise on Mutations, SSRs/markers (effects on Transcription &Translation )

Jaiswal/ Austin, Matt Noor, Parul

 

 

Day-5

Friday

July 1, 2016

9:00 - 9:05

1:00 - 1:05

Review of Day 4 & Questions?

Chang, Matt

Cordley 3089

 

9:05-10:00

1:05 - 2:00

Visualize the amplified DNA fragment on a gel find genotype differences

Jaiswal / Matt, Noor

Visualize results of genetic transformation of plants and Group Discussion

 

Chang / Elizabeth

10:00 - 10:15

2:00 - 2:15

Break and Snacks

 

Cordley 3086

10:15 - 12:00

2:15-4:00

Informatics- based analysis for DNA and Protein in biology -webtools

Jeff Anserson, Justin,

Noor, Matt, Austin

Cordley 4056

12:00 - 12:45

N/A

PIZZA Lunch (for both morning and afternoon participants)

Meet Scientists, Questions, Certificates, Conclusion

All folks (week-1) + campers

Cordley 2087

 

N/A

4:00pm

Pickup boxed dinner (only afternoon camp) and depart for Portland

 

Cordley 3089