Replicate 1: (morning camp)
Check-in: 8:45 - 9:00am Everyday
Check-out: 12:00pm Everyday
Replicate 2: (Afternoon camp)
Check-in: 12:45-1:00pm Everyday
Check-out: 4:00pm Everyday
Instructors:
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Week-1 |
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● Pankaj Jaiswal ● Justin Preece |
● Jeff Chang ● Alexandra Weisberg |
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● Sushma Naithani |
● Jeff Anderson ● Megan O’Malley |
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● Ramesh Sagili ● Hannah Lucas |
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SCHEDULED ACTIVITIES
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Day & Date |
Morning Camp |
Afternoon Camp |
Activity |
Lead / Instructors |
Location |
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Day-1 Mon June 27, 2016 |
9:00 - 9:15 |
1:00 - 1:15 |
Camp Overview |
Jaiswal |
Cordley 3089 |
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9:15 - 9:45 |
1:15 - 1:45 |
Safety and Pipetting (using dyes) |
Jaiswal/ Matt |
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9:45 -10:45 |
1:45 -2:45 |
Central Dogma and Genetic Material (hands on activity on DNA modeling exercise, transcription and translation)
|
Jaiswal/ Noor, Matt Austin, Parul |
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10:45 - 11:00 |
2:45 - 3:00 |
Break and Snacks |
|
Cordley 3086 |
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11:00 - 12:00 |
3:00 - 4:00 |
Talk to them about ...What they are doing and why?
Genetic transformation of experimental model, tobacco plants, with T-DNA inserts carrying GUS reporter gene (GMOs). If time permits use the time to discuss GMOs |
Chang / Elizabeth |
Cordley 3089 |
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Day-2 Tue June 28, 2016 |
9:00 - 9:05 |
1:00 - 1:05 |
Review of Day 1 & Questions? |
Jaiswal |
Cordley 3089 |
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9:05 - 9:45 |
1:05 - 1:45 |
Pipetting (lesson two) |
Jaiswal / Matt, Noor, Parul, Austin |
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9:45 - 10:45 |
1:45 - 2:45 |
Plant DNA isolation |
Jaiswal / Matt, Noor, Parul, Austin |
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|
10:45 - 11:00 |
2:45 - 3:00 |
Break and Snacks |
|
Cordley 3086 |
|
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11:00 -12:00 |
3:00 - 4:00 |
Bacterial DNA isolation |
Chang / Alex, Ed |
Cordley 3089 |
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Day-3 Wed June 29, 2016 |
9:00 -9:05 |
1:00 -1:05 |
Review of Day 2 & Questions? |
Alex, Matt, Noor |
Cordley 3089 |
|
9:05 - 9:30 |
1:05 - 1:30 |
Polymerase Chain Reaction (PCR). Amplify genetic markers (SSR) containing DNA segment from various varietis of rice. |
Jaiswal / Matt, Noor, Parul, Austin |
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9:30 - 10:15 |
1:30 - 2:15 |
Preparation of bacterial DNA library for whole genome sequencing |
Chang / Alex & Ed |
Cordley 3089 |
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10:15 - 10:30 |
2:15 - 2:30 |
Break and Snacks |
|
Cordley 3086 |
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10:30 - 12:00 |
2:30 - 4:00 |
Genetic Markers and Phenotypes (Hands on activity on Mendelian Genetics) |
Naithani/ Noor, Matt, Parul, Austin |
Cordley 3089 |
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Day-4 Thu June 30, 2016 |
9:00 - 9:05 |
1:00 -1:05 |
Review of Day 3 & Questions? |
Alex, Matt, Austin |
Cordley 3089 |
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9:05 - 9:30 |
1:05 - 1:30 |
GUS reporter gene assay for genetic transformation of tobacco plants (continue from Day-1) |
Chang / Elizabeth |
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9:30 - 10:00 |
1:30 - 2:00 |
PCR gel loading |
Jaiswal / Matt, Noor, Parul, Austin |
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|
10:00 - 10:30 |
2:00 - 2:30 |
Tour of the Center for Genome Research and Biocomputing (CGRB)- Form 2 groups |
Jaiswal (group-1) / Noor (group-2)/ Parul |
ALS-CGRB |
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10:30 - 10:45 |
2:30 - 2:45 |
Break and Snacks |
|
Cordley 3086 |
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10:45 - 12:00 |
2:45 - 4:00 |
DNA Modeling Exercise on Mutations, SSRs/markers (effects on Transcription &Translation ) |
Jaiswal/ Austin, Matt Noor, Parul |
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Day-5 Friday July 1, 2016 |
9:00 - 9:05 |
1:00 - 1:05 |
Review of Day 4 & Questions? |
Chang, Matt |
Cordley 3089
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9:05-10:00 |
1:05 - 2:00 |
Visualize the amplified DNA fragment on a gel find genotype differences |
Jaiswal / Matt, Noor |
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Visualize results of genetic transformation of plants and Group Discussion
|
Chang / Elizabeth |
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10:00 - 10:15 |
2:00 - 2:15 |
Break and Snacks |
|
Cordley 3086 |
|
|
10:15 - 12:00 |
2:15-4:00 |
Informatics- based analysis for DNA and Protein in biology -webtools |
Jeff Anserson, Justin, Noor, Matt, Austin |
Cordley 4056 |
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12:00 - 12:45 |
N/A |
PIZZA Lunch (for both morning and afternoon participants) Meet Scientists, Questions, Certificates, Conclusion |
All folks (week-1) + campers |
Cordley 2087 |
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N/A |
4:00pm |
Pickup boxed dinner (only afternoon camp) and depart for Portland |
|
Cordley 3089 |